Intuitive method to assist you to find the right chromatography column for your personal biopharmaceutical applications.
Spectral bandwidth: Even though the user could choose a specific wavelength, the actual assortment is made of a range of wavelengths that passes throughout the flow mobile along with the exit slit.
Learn how a batch procedure for mAb purification may be steadily remodeled into an intensified process and after that into a linked procedure.
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When only solvent is passing in the sample part the calculated refractive index of both equally parts is similar, but when an analyte passes from the move mobile The 2 calculated refractive index are different.
Twin LC procedures are valuable for any situation when you must improve your sample throughput, like examining a sample for residual pesticides here and phenolic information in just one run or performing replicate analyses simultaneously.
New software spots are launched via the LC-MS system thanks to its pros concerning sensitivity and determination.
Trap methods allow for pre-concentration of reduced-abundant analytes and address solvent incompatibility issues before the portion is eluted onto a second dimension (2D) column to resolve tricky or co-eluting peaks.
Detectors that may detect all compounds, like a refractive index detector and an evaporative light scattering detector, are called “universal detectors”.
Common C-Mount Adapter: An adapter made to connect cameras and spectrophotometers to some microscope photoport so that they're parfocal and parcentral Using the eyepieces.
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Gradient methods include things like a modify in the cellular period composition across a separation. These methods often hire two solvents, named A and B.
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Cellular period begins to movement. The pump pushes the eluents or solvents with the procedure in a specified move rate.